chicken polyclonal anti-bdnf (Promega)
90
Structured Review
Promega
chicken polyclonal anti-bdnf
Chicken Polyclonal Anti Bdnf, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chicken+polyclonal+anti-bdnf/chicken+anti+bdnf/pmc07725275-15-0-4
Average 90 stars, based on 1 article reviews
Chicken Polyclonal Anti Bdnf, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chicken+polyclonal+anti-bdnf/chicken+anti+bdnf/pmc07725275-15-0-4
Average 90 stars, based on 1 article reviews
chicken polyclonal anti-bdnf - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss"
Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss
Journal: Cell reports
doi: 10.1016/j.celrep.2018.09.077
Figure Legend Snippet: KEY RESOURCE TABLE
Techniques Used: Virus, Recombinant, Transfection, Western Blot, Sequencing, Plasmid Preparation, Expressing, Software
Related Articles
Virus:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Recombinant:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Transfection:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Western Blot:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Sequencing:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Plasmid Preparation:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Expressing:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Software:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Immunostaining:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Avidin-Biotin Assay:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Incubation:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Immunodetection:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Fluorsave:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Double Immunostaining:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Marker:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with Labeling:Article Title: RbAp48 Protein Is a Critical Component of GPR158/OCN Signaling and Ameliorates Age-Related Memory Loss Article Snippet: Article Title: UPREGULATION OF BRAIN-DERIVED NEUROTROPHIC FACTOR EXPRESSION IN NODOSE GANGLIA AND THE LOWER BRAINSTEM OF HYPERTENSIVE RATS Article Snippet: Every other section (i.e. one series) was processed for BDNF immunostaining with Article Title: Upregulation of brain-derived neurotrophic factor expression in nodose ganglia and the lower brainstem of hypertensive rats. Article Snippet: Every other section (i.e., one series) was processed for BDNF immunostaining with Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Immunostaining of sections Sections were (1) incubated for 1 h in a 10% solution of goat serum in dilution buffer (DB; 0.02 M phosphate buffer, 0.5 M NaCl, 0.3% Triton X-100), (2) incubated for 2 h in chicken polyclonal anti-BDNF [1:50 (NGs) or 1:25 (brainstems); Promega, Madison, WI] in DB, applied alone, or in combination with either rabbit polyclonal anti-HCN1 (1:100; Alomone Labs, Jerusalem, Israel) or rabbit polyclonal anti-VR1 (TRPV1), N-terminus (1:500; Neuromics, Edina, MN), (3) washed three times in DB, (4) incubated in secondary antibody diluted in DB with 10% goat serum as specified for the following types of immunostaining: (i) NG, immunodetection of BDNF only, 1 h in goat anti-chicken biotinylated IgG (1:200, Vector Laboratories, Burlingame, CA), (ii) brainstem, immunodetection of BDNF only, 2 h in donkey anti-chicken IgG-Cy2 (1:200, Jackson Immunoresearch; West Grove, PA), (iii) NG, double immunodetection of either BDNF/HCN1 or BDNF/VR1 (TRPV1), 1 h in goat anti-chicken IgG-Alexa 488 (1:1000, Invitrogen, Carlsbad, CA) and goat anti-rabbit IgG-Alexa 647 (1:1000, Invitrogen, Carlsbad, CA), (5) washed three times in PBS, (6) incubated for 30 min in avidin-biotin reagent (ABC) in PBS-0.5M NaCl (1:100, Vectastain Elite , Vector Laboratories;), (7) washed for 10 min in PBS-0.5M PBS, (8) washed two times in PBS, (9) incubated for 3–5 min in diaminobenzidine (DAB) solution (in PBS: 0.3 mg/ml DAB, 0.032% NiCl 2 , 0.0075% H 2 O 2 ), (10) washed three times in PBS, and (11) mounted with Gel Mount (Sigma) or FluorSave (Calbiochem, San Diego, CA). Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: HRP was activated with chemiluminescent reagents (Western Lightning, Perkin-Elmer, Waltham, MA) for 90 sec, and blue X-ray film (Phenix Research, Candler, NC) was exposed for 2 min. Article Title: Brain-Derived Neurotrophic Factor in Arterial Baroreceptor Pathways: Implications for Activity-Dependent Plasticity at Baroafferent Synapses Article Snippet: Double-immunostaining of cultures for BDNF and Neurofilament, a pan-neuronal marker Cultures were (1) incubated for 1 h in 1:1 solution of goat serum and PBS-0.1% Triton X-100, (2) incubated for 2 h in Article Title: TOOTH PULP INFLAMMATION INCREASES BDNF EXPRESSION IN RODENT TRIGEMINAL GANGLION NEURONS Article Snippet: Double-immunohistochemistry was performed as previously described ( Buldyrev et al., 2006 ; Martin et al., 2009 ) with |
![Effect of BDNF on NEFA levels before and after treatment to GK rats in fed and fasted conditions. Plasma NEFA levels in fed and 12 h-fasted conditions at time points before treatment (Day –1/0), immediately after termination of treatment (Day 6/7), and 4/5 days after termination of treatment (Day 10/11) were measured. Plasma NEFA level was higher in fasted condition at all time points measured in all groups [Pre(Day –1/0), p < 0.05 vs. fed conditions, Two-Way ANOVA with Tukey's post-hoc test]. At time point immediately after termination of treatment (Day 6/7), plasma NEFA levels in Wistar-BDNF and GK-BDNF groups were increased both at fed and fasting conditions. Especially, plasma NEFA levels in Wistar-BDNF and GK-BDNF groups at fasting condition were significantly higher than those in GK-Vehicle group at fasting condition ( * p < 0.05, Two-Way ANOVA with Tukey's post-hoc test). Such tendency continued to Day 10/11 but there was no significant difference at this time point.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8348/pmc03788348/pmc03788348__fnsyn-05-00007-g0008.jpg)
